Potential of CRISPR/Cas system as emerging tools in the detection of viral hepatitis infection

Table 3 Applications of CRISPR/Cas system in viral hepatitis detection

Types of Hepatitis	Platform Name	Cas Protein	Amplification Methods	Visualization	Sensitivity	Times	References
HBV	CRISPRHBV	Cas12b	MCDA	Real-time/ Fluorescence	10 copies/µL	60 min	[91]
	-	Cas13a	RCA/PCR	Fluorescence	1 copy/µL	-	[92]
	PCR-CRISPR	Cas13a	PCR	Fluorescence	1 copy/µL	15 min	[93]
				Fluorescent		13 min
	LAMP-Cas12a-based HBV	Cas12a	LAMP	Lateral flow test strips	1 copy/µL	20 min	[94]
HCV	RT-LAMP-Coupled CRISPR–Cas12	Cas12a	RT-LAMP	Fluorescent/ Lateral flow test strips	10 ng/µL	-	[97]

ISSN: 1743-422X