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Fig. 3 | Virology Journal

Fig. 3

From: Independent role of caspases and Bik in augmenting influenza A virus replication in airway epithelial cells and mice

Fig. 3

Pan-caspase inhibitor diminished IAV replication in mouse airway epithelial cells (MAECs). (A) Differentiated MAECs were infected with 0.1 MOI IAV (H1N1) PR/8/34 strain and treated with vehicle control or 20 µM of the pan-caspase inhibitor, Q-VD-Oph. The inhibitors were added on apical and basal sides 3 h prior to viral infection. The inhibitors were removed from the apical side but were kept with media in the basal side for the whole experimental period. Protein lysates were analyzed for caspase 3 cleavage by Western blot. The infectious viral titers were analyzed from apical washes of the cells 72 h post-infection in (B) bik+/+ cells, (C) bik−/− cells. Protein lysates were analyzed for the expression of Bik by Western blotting. Experiments were performed in triplicates and the immunoblots are representative of at least three separate experiments. Error bars indicate mean ± SEM (n = 6/group for all experiments; N = 2); p < 0.05 was considered significant

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