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Table 6 Primer sequences for Dual-luciferase reporter gene assay

From: Screening and identification of cyprinid herpesvirus 2 (CyHV-2) ORF55-interacting proteins by phage display

PCR

Luciferase

Gene Name

Nucleotide sequence(5’-3’)

Amplicon size(bp)

1

pEGFP-N1

ORF55

F:GGACTCAGATCTCGAGATGGCTTTTCTGGAGTTGGTGC

645

R:GGCGACCGGTGGATCCCGTACAAAAGAACAAGGGGCATCCG

2

pDsRed-Express-N1

ABRA

F:TACCGGACTCAGATCTCGAGATGGAGACAGACGAAGCACC

474

R:TGGTGGCGACCGGTGGATCCCGCTGTAACAAGGTGATGAGCACT

3

pEGFP-N1

GFP

F:CGCAAATGGGCGGTAGGCGTG

227

R:CGTCGCCGTCCAGCTCGACCAG

4

pDsRed-Express-N1

RFP

F:ATAGCGGTTTGACTCACGGG

585

R:GCCGTCCTCGAAGTTCATCA

  1. Based on the sequences of ORF55 and ABRA provided by NCBI, common PCR primers were successfully designed and applied to subsequent ligation of dual-fluorescein reporter vectors. Universal primers used for sequencing were also designed