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Table 5 Oligonucleotide primers and conditions for Real-time PCR

From: Screening and identification of cyprinid herpesvirus 2 (CyHV-2) ORF55-interacting proteins by phage display

RT-PCR

Gene Name

Nucleotide sequence(5’-3’)

Amplicon size(bp)

Tm(℃)

1

ZFP

F:CCGTTCACCTGCCATCATTG

74

60

R:ACATGACGGCTCACATGACT

2

WDR7

F:ATCACTGCAGGCTGTCTGTC

242

60

R:AGGGCTTTACAGGTGTGTCG

3

ZFP516

F:TGATGCCATGGCGAGTATCC

152

60

R:TAGGCCGGTACTCAGTGTCA

4

ABRA

F:GCACCGTATCCGTCAAAGGT

102

60

R:GAGTCACCTTGCCATTGCTG

5

β-actin

F:CACTGTGCCCATCTACGAG

224

55

R:CCATCTCCTGCTCGAAGTC

  1. Based on the results of the reciprocal polypeptide screen, complete nucleic acid sequences were searched from NCBI and RT-qPCR primers were designed separately. The β-actin gene refers to the housekeeping gene in the relative quantification