Molecular epidemiology analysis of symptomatic and asymptomatic norovirus infections in Chinese infants

Table 1 Mapping of immunogenic sites within VP1 region of different GII.4 strains in this study

Epitopes with mutations	Mutation Sites	Position of VP1 ^b	Mutation Effect ^c	PolyPhen-2 Score ^c
T cell restricted in immunodominant epitopes
SPSQVTMFPHIIVDVRQL_134–151	I145V	Shell	Benign	0.356
VRNNFYHYNQSNDST_161–175	S174P	Shell	Benign	0.010
GRNTHNVHL_410–418	H414P ^a	P2	Damaging	0.699
B cell restricted in immunodominant epitopes
GDVTHITGSRNYTMNLASQNWSNY_288–311	G295C	P2	Damaging	0.972
GDVTHITGSRNYTMNLASQNWSNY_288–311	S309N	P2	Benign	0.05
Antibody epitopes
Epitope A_{294–298, 368, 372–373}	N7373H	P2	Benign	0.237
Epitope D_393–397	G393S	P2	Benign	0.143
Epitope E_{407, 411–414}	H414P ^b	P2	Damaging	0.699
Other single amino acid substitutions
N/A	V8A	N	Damaging	0.571

CD4 + T restricted are in purple font, CD8 + T restricted are in blue font
Protein site information is referenced to GII.4 Sydney 2012 VP1 (AFV08795)
^aIndicated residue targeted by both antibody and T cell receptor response
^bN-terminal:1–45; Shell domain: 46–221; P1 subdomain: 222–275 & 418–540; P2 subdomain: 276–417
^cResults from PolyPhen-2. Score goes from 0 to 1. Greater score indicates higher probability to impair the protein function