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Fig. 7 | Virology Journal

Fig. 7

From: ARIH1 inhibits influenza A virus replication and facilitates RIG-I dependent immune signaling by interacting with SQSTM1/p62

Fig. 7

ARIH1 did not affect the K63-linked polyubiquitin and oligomerization of RIG-I. A HEK293T cells were co-transfected with pFlag-ARIH1 and HA-tag RIG-I-N plasmid (pHA-RIG-I-N) plus pMyc-UbWT, pMyc-UbK63 or pMyc-UbK63R. Immunoprecipitation assay was performed using HA tag antibody. The polyubiquitination of RIG-I was detected by Myc labeled antibody. B HEK293T cells were co-transfected with pHA-RIG-I, FLAG-tag RIG-I plasmid (pFLAG-RIG-I), and Myc-tag ARIH1 plasmid (pMyc-ARIH1) or their corresponding empty vectors, and the effect of ARIH1 on the interaction between HA-tag RIG-I and FLAG-tag RIG-I was detected by immunoprecipitation assay using Flag tag antibody

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