Fig. 4From: ARIH1 inhibits influenza A virus replication and facilitates RIG-I dependent immune signaling by interacting with SQSTM1/p62ARIH1 increased phosphorylated IRF3 level. A HEK293T cells were stimulated with SeV 24Â h after transfection with pFlag-ARIH1 or empty vector. Western blot assay was performed using antibodies specific for Flag, p-IRF3, IRF3, and GAPDH. B HEK293T cells were transfected with siARIH1 to silence ARIH1, and siNC was used as control. After 36Â h, SeV was used for stimulation, and Western blot assay was performed using antibodies specific for ARIH1, p-IRF3, IRF3, and GAPDH. C A549 cells were stimulated with SeV 48Â h after transfection with pFlag-ARIH1 or empty vector. Western blot assay was performed using antibodies specific for Flag-tag, p-IRF3, IRF3, and GAPDH. D A549 cells were transfected with siARIH1 to silence ARIH1, and siNC was used as control. After 36Â h, SeV was used for stimulation, and Western blot assay was performed using antibodies specific for ARIH1, p-IRF3, IRF3, and GAPDHBack to article page