Fig. 3

Effect of ARIH1 on influenza A virus internalization and polymerase activity. A A549 cells were transfected with siARIH1^#1 or siNC for 36 h, and then incubated with H1N1/PR8 for 0 or 45 min. Cells were lysed after thorough washing with PBS buffer, and virus internalization was detected by Western blot assay. B Polymerase activity was assayed, and each experiment was independently repeated three times. Data are presented as means ± SD from three independent experiments. ns, P ≥ 0.05 as determined by student’s t test