Fig. 1
ARIH1 conferred cellular resistance to H1N1/PR8 infection. A A549 was transfected with siARIH1#1, siARIH1#2 or siNC for 36 h, and the relative M1 and NP expression of H1N1/PR8-infected cells at 24 hpi was measured by Western blot assay. B TCID50 assay was also performed to measure the titer of the culture supernatant of the above cells at 12 and 24 hpi. Data are presented as means ± SD from three independent experiments. *, P < 0.05 and **, P < 0.01 as determined by student’s t test. C Plasmid expressing Flag-tag ARIH1(pFlag-ARIH1) or its vector was transfected in A549 cells. After 24 h, the cells were infected with H1N1/PR8 for 12 or 24 h and the cell lysates were analyzed by Western blot assay using antibodies specific for Flag-tag, M1, NP, and GAPDH. D Amount of progeny virus in the supernatant from treated cells determined by TCID50 assay. Data are presented as means ± SD from three independent experiments. **, P < 0.01 as determined by student’s t test