Fig. 6
From: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions
Western-blot analysis of polysomal fractions. The representative images of A254 profiles (as in Fig. 4A) and western blots of 60S ribosomal protein L7a (RsL7a), eIG4G, eIF4A, Strep-Nsp2, eIF4E2, and 4EBP in corresponding fractions (Frs) along sucrose gradients in control and Nsp2 cells grown under normal (A) and hypoxic (B) conditions. Arrows on the A254 profiles pont to the picks representing 80S. The western blot analysis of proteins was investigated in three sucrose density gradients using lysates from separate experiments. Arrow, position of Nsp2 signal