Fig. 5
From: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions
Western blot analyses of FGF2 and VEGF proteins in lysates. A FGF2 CUG1 and AUG isoforms in Nsp2 expressing cells in normoxic and hypoxic conditions (Right and left panel represents three biologically independent experiments). GAPDH was used as a normalization control. B Densitometric ratio of CUG1 to GAPDH (left panel) and AUG to GAPDH (right panel). C Left, the representative images of western blots of VEGF-C, EGFR, and GAPDH. Right, graphs of the densitometric analysis of western blots. The graphs represent the mean ratio of signal of VEGF-C to GAPDH normalized to that in control cells grown under normal conditions, set as one (± SD). Western blot analysis was performed for each set of proteins using lysates from three separate experiments and repeated at least twice for each set of lysates