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Fig. 4 | Virology Journal

Fig. 4

From: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions

Fig. 4

Polysomal analysis of control and Nsp2-cells in sucrose density gradients. A The representative of A254 profiles representing lysates distribution of cells grown under normal (left panel) and hypoxic (right panel) conditions in sucrose density gradients. Top of the gradient is on left side of each image. Position of 40S, 60S, and 80S along Ps profile are indicated. Arrow, 80S pick. L, light polysomes representing low translated mRNAs, fractions #1–6; H, heavy polysomes representing more efficiently translated mRNAs, fractions # 7–11. Ps/Ms, ratio of the 18S RNA signals in polysomes versus monosomes (fractions #4 and 5). B Real-time-PCR analysis of GAPDH, FGF2, and VEGF-C mRNAs distribution in sucrose density gradients. Left panels, distribution of the corresponding mRNA along sucrose density gradient similar to ones presented on (A). Graphs represent percentage of individual mRNA in each fraction. Filled circle, control cells; empty circle, Nsp2-cells. Right panels, graphs representing percentage of individual mRNA in light polysomal fractions #1–6 (white bars) and in heavy polysomal fractions #7–11 (grey bars) relative to the amount of this mRNA in all eleven fractions. The graphs represent the mean value from three independent polysomal ultracentrifugations (± SD)

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