Fig. 2
From: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions
Nsp2 co-localizes with eIF4E2 and RER under normal and hypoxic conditions. A Strep-Nsp2 was probed using anti-strep antibody (green) and eIF4E2 probed using anti-eIF4E2 antibody (red), DAPI (blue) stained nuclei. Merge channel from max intensity projections of z-stacks under hypoxia shows increased co-localization (yellow). B Strep-Nsp2 (green) and calnexin (red) probed. Under hypoxia, granular staining in merged channel (yellow) shows increased co-localization from best focal plane. C Representative image of Ribosomal marker (RbS3) probed using anti-RbS3 antibody (red) and Strep-Nsp2 (green). Colocalization shown in merged channel. DAPI stained nuclei