Fig. 1
From: SARS-CoV-2 viral protein Nsp2 stimulates translation under normal and hypoxic conditions
Expression of Nsp2 and its binding to eIF4E2. A Representative image of western blot analysis of expression of Strep-Nsp2 in HEK293T cells stably transfected with either Lipofectamine-3000 or LTX. B Biotin-Agarose pull-down. Left, western blot analyses of eIF4E2 (upper panel) and Nsp2 (lower panel). Right, graph represents densitometric ratio of eIF4E2 to Nsp2. C m7GTP-Sepharose pull-down. Left, western blot analyses of Nsp2 (upper panel) and eIF4E2 (lower). Right, graph represents densitometric ratio of Nsp2 to eIF4E2. C—control; N—normoxia; H—hypoxia. The blots shown are representative of three completely independent experiments