Skip to main content

Table 6 The dual ERA optimal reaction conditions

From: Development and application of a dual ERA method for the detection of Feline Calicivirus and Feline Herpesvirus Type I

Parameters

Amplification conditions

Temperature /℃

42

FHV-1 probe volume/µL

0.3

FHV-1 primer volume/µL

1.6

FCV probe volume/µL

0.3

FCV primer volume/µL

2.6

Activator/µL

2

Deliquescent agent/µL

20

Template volume /µL

2

ddH2O volume /µL

21.2