From: Development and application of a dual ERA method for the detection of Feline Calicivirus and Feline Herpesvirus Type I
Parameters
Amplification conditions
Temperature /℃
42
FHV-1 probe volume/µL
0.3
FHV-1 primer volume/µL
1.6
FCV probe volume/µL
FCV primer volume/µL
2.6
Activator/µL
2
Deliquescent agent/µL
20
Template volume /µL
ddH2O volume /µL
21.2