Molecular characterization of coxsackievirus B5 from the sputum of pneumonia children patients of Kunming, Southwest China

Table 4 Changes and prediction of amino acid substitution effect of VP1 protein from five CVB5 isolates

VP1 amino acid residue		Prototype strain residue	Substitution	PROVEAN prediction
Position	Location	Prototype strain residue	Substitution	PROVEAN prediction
3	N-terminus	P	P3T	Neutral
7	N-terminus(α-helix)	I	I7V	Neutral
75	β pleated sheet	Y	Y75F	Deleterious
80	BC-loop	K	K80R	Neutral
90	BC-loop	A	A90G	Neutral
91	β pleated sheet	Q	Q91Y	Neutral
125	DE-loop	S	S125T	Neutral
132	DE-loop	K	K132Q	Neutral
140*	β pleated sheet	T	T140I	Deleterious
166*	EF-loop	N	N166T	Deleterious
200	β pleated sheet	R	R200K	Neutral
268	C-terminus	S	S268T	Neutral
273	C-terminus	G	G273S	Neutral

Position Amino acid mutation site of VP1 protein compared to the prototype strain (Faulkner). Location The position of amino acid mutation site in VP1 protein secondary structure. BC Loop, DE-loop and EF-loop: The loop of antigen-antibody interaction. Prototype strain residue The amino acid residue in the prototype strain. PROVEAN prediction The PROVEAN algorithm result for the amino acid residue substitution
*It was not the common mutation site of the five isolates

ISSN: 1743-422X