Table 2 Primers for amplifying the complete genome sequence of CVB5 strains
Primer | Position | Sequence (5´ ~ 3´) | Orientation |
|---|---|---|---|
CVB5-F1 | 1–20 | TTAAAACAGCCTGTGGGTTG | Forward |
CVB5-R1 | 461–480 | AGTTGGGATTAGCCGCATTC | Reverse |
CVB5-F2 | 388–407 | ATTYCGACATGGTGCGAAGA | Forward |
CVB5-R2 | 948–967 | TCCTCTGCTGACGGAGAGTT | Reverse |
CVB5-F3 | 909–928 | TCAARTCSATGCCTGCYCTC | Forward |
CVB5-R3 | 1504–1523 | RTTGATCCACTGRTGCGGGA | Reverse |
CVB5-F4 | 1459–1478 | GGTTGGWGTYGGCAATCTGA | Forward |
CVB5-R4 | 2131–2150 | AGCHCCTGGTGGTGARTACG | Reverse |
CVB5-F5 | 2105–2124 | AATGGCAACRGGYAAATTCC | Forward |
CVB5-R5 | 2939–2958 | AGAACACRCTAGGGTTGGTG | Reverse |
CVB5-F6 | 2856–2875 | TCATGTATGTGCCCCCGGGT | Forward |
CVB5-R6 | 3581–3600 | CGGCAAGCAARACATGTGTC | Reverse |
CVB5-F7 | 3453–3472 | CAACCGGGGTGTATTTYTGY | Forward |
CVB5-R7 | 4254–4273 | GCACTTTGCTCAATGGTGGC | Reverse |
CVB5-F8 | 4134–4153 | TACCGGARGTGARGGAGAAG | Forward |
CVB5-R8 | 4893–4912 | ATGGCCTTYCCRCACACGAG | Reverse |
CVB5-F9 | 4801–4820 | RRTCAACATGCCCATGTCAG | Forward |
CVB5-R9 | 5746–5765 | TCTCTTYGTGGGRGTGCCAC | Reverse |
CVB5-F10 | 5642–5661 | GCTGTYYTRGCWATAAACAC | Forward |
CVB5-R10 | 6463–6482 | TGTYTGYCTCATTGCHACWG | Reverse |
CVB5-F11 | 6398–6417 | CAGRTCWGCDGARAAGGTGG | Forward |
CVB5-R11 | 7384–7403 | CCGCACCGAATGCGGAGAAT | Reverse |