Fig. 4
From: Inducible nitric oxide synthase deficiency promotes murine-β-coronavirus induced demyelination
Early demyelination in the absence of NOS2 is an effect of engulfment of MBP and PLP by Iba1 + MG/Mφ. (A) to (B) are representative epifluorescence scans of spinal cord sections and respective confocal photomicrographs. 5 μm thick transverse sections of paraffin embedded spinal cords from infected WT and NOS2-/- mice at day 9/10 p.i. were double immunolabelled for MBP (green) with Iba1 (red) and PLP (green) with Iba1 (red) and counterstained with DAPI (blue). Insets highlight areas that show engulfment of MBP or PLP by Iba1 + MG/Mφ. Scale bar 100 μm for epifluorescence image and 10 μm for confocal image