Virome of wild rats (Rattus norvegicus) captured far from pig farms in Jiangsu province of China reveals novel porcine circovirus type 2d (PCV2d) sequences

Table 1 The primers of nested PCR used for detection and amplification of the PCV2 genome

Fragment	Primer name	Application	Primer sequence (5′–3′)
1	1WF/1NF	1st and 2nd round	TGCTGTGAGTACCTTGCTGG
1	1WR	1st round	CCGTGGATTGTTCTGTAGCA
1	1NR	2nd round	GTAGATCATCCCAGGGCAGC
2	2WF^a	1st round	TGCTGTGAGTACCTTGCTGG
2	2WR^a	1st round	CCATCTTGGCCAGATCCTCC
2	2NF^a	2nd round	GCAGACCCGGAAACCACATA
2	2NR^a	2nd round	GAATGTGGACATGATGAGAT
3	3WF	1st round	GGGTTATGGTATGGCGGGAG
3	3WR	1st round	CAAACGTTACAGGGTGCTGC
3	3NF	2nd round	ATAACAGCAGTGGAGCCCAC
3	3NR	2nd round	CCAGCAAGGTACTCACAGCA

^aThe primers were also used to detect the tissue distribution of PCV2 in wild rats

ISSN: 1743-422X