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Fig. 5 | Virology Journal

Fig. 5

From: Proteins in pregnant swine serum promote the African swine fever virus replication: an iTRAQ-based quantitative proteomic analysis

Fig. 5

Validation of DEPs between PSS and NPSS based on iTRAQ technology. A The MASP1, PCNA, SERPINB2, SLA-1, VTN, JUNB, SFXN1, PM20D1, BST2, and SEC14L1 were randomly selected from the upregulated or downregulated DEPs to verify changes in their mRNA transcript levels; GAPDH was used as an internal reference to determine iTRAQ feasibility using the 2−∆∆CT normalization method. B iTRAQ accuracy was verified via western blot using the randomly selected upregulated proteins PCNA, JUNB, and MASP1 and the downregulated protein BST2. The analysis of gray values in the figures was performed using the ImageJ software

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Virology Journal

ISSN: 1743-422X