Fig. 1

PSS promoted ASFV replication. A and B BMDMs were cultured in RPMI 1640 medium with 10% NPSS and 10% PSS for 24 h; then they were infected with ASFV (MOI = 0.5). Samples were collected 24 h after infection. The cell supernatant was collected in duplicate, and ASFV genomic copies were detected using absolute quantitative analysis after repeated freezing and thawing for three times. Another sample was used for western blot to detect protein expression of ASFV P72 and P30, using β -actin as the control. The gray value analysis was performed using the ImageJ software. Experimental data are expressed as the mean ± standard deviation values of three independent experiments. *** indicating p values of < 0.001 was considered statistically significant