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Table 3 Overview of major nucleotide calling results used to generate consensus genome sequences in eight HPV replicates

From: TaME-seq2: tagmentation-assisted multiplex PCR enrichment sequencing for viral genomic profiling

Sample ID

# of identically called major nucleotides in Run1 and Run2

# of major nucleotides called only in

# of major nucleotides called differently between runs

Mean coverage of the major nucleotide

Run1

Run2

Run1

Run2

1-HPV51

7766

1

6

0

27,711

8886

2-HPV52

7941

0

0

0

5429

2869

3-HPV52

7942

0

0

0

50,585

29,773

4-HPV51

7788

1

6

0

71,638

13,617

5-HPV52

7941

1

0

0

5816

1372

6-HPV52

7942

0

0

0

8474

4117

7-HPV51

7787

3

2

0

52,371

8405

8-HPV52

7942

0

0

0

82,431

32,528

  1. For each pair of samples, the number of identical major nucleotides between runs, the number of nucleotides called in one of the runs, and the number of called nucleotides differing between runs are shown. The mean coverage of the called major nucleotides in two runs is also presented