From: TaME-seq2: tagmentation-assisted multiplex PCR enrichment sequencing for viral genomic profiling
Virus type | # of samples | Mean # of raw reads (mil) | Mean # of trimmed reads (mil) | Mean # of reads mapped to target (mil) | Mean % raw reads mapped to target (%) | Mean % trimmed reads mapped to target (%) | Mean coverage per sample | Mean % of genome covered by minimum | Variable sites in samples passing 300× mean coverage | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
10x (%) | 100x (%) | Mean | Min | Max | Mean # of variable sites per 1 kb of genome | ||||||||
HPV51 | 12 | 22.7 | 9.0 | 2.7 | 15.5 | 37.3 | 24,911 | 89.4 | 83.4 | 21 | 4 | 53 | 2.7 |
HPV52 | 12 | 10.4 | 5.4 | 1.3 | 8.5 | 18.1 | 13,003 | 75.9 | 63.7 | 19 | 3 | 38 | 2.4 |
HPV59 | 12 | 8.6 | 3.4 | 2.0 | 16.2 | 38.2 | 14,893 | 74.6 | 57.9 | 20 | 5 | 30 | 2.5 |
SARS-CoV-2 | 23 | 23.0 | 18.2 | 1.3 | 5.4 | 12.5 | 3454 | 43.3 | 28.7 | 116 | 24 | 215 | 3.9 |