Fig. 5
From: Interaction of the Zika virus with the cytoplasmic dynein-1
Coomassie-stained PAGE-SDS gel showing binding of the ZIKV with NTDyn. Purified ZIKV from Vero cells was loaded into a 5 ml HisTrap HP Nickel (GE) column pre-loaded with the His6NTDyn. The column was washed, and the sample was eluted with imidazole. The fractions were analyzed by SDS-PAGE. Lane 1. MW marker. Lane 2. The purified His6NTDyn band. Lane 3. Enriched pE from the ZIKV. Lane 4. The eluted fraction contains both, the complete ZIKV and NTDyn-His. Lane 1. MW marker. Lane 2. His6NDD purified protein bound to affinity column, Lane 3. Purified ZIKV. Lane 4. Elution, C. Lane 1. MW marker. Lane 2. His6NTDyn + anti-NDD antibody complex. Lane 3. Purified ZIKV. Lane 4. Elution. D. Lane 1. MW marker. Lane 2. Purified ZIKV. Lane 3. Elution. E. left panel, the structure of the blue NTDyn is shown, composed in its nucleus by the NDD and in its external part by 6 lateral alpha-helices. Modified images of 5NW4 [14] and 5OWO [15]. F. NDD. Dimerization domain of dynein in red color. Modified images of 5OWO