Fig. 2

Proximity ligation assay of ZIKV infected cells. A (M) Mock image is cells without infection fixed at 48 h C control was cells infected with the virus at 18 h without the incubation of the anti-pE antibody. For the time course, we used Vero cell infected with ZIKV and fixed at different times post-infection: 12, 18, 24, and 48 h This assay was performed with a monoclonal anti-pE in muse MAb and with a polyclonal anti- NTDyn in rabbit. The PLA dots were marked with Cy3 (red). The images on the right show DAPI (blue) and Cy3 (red) channels. The images on the left show blue, red and the differential interference contrast channel (grey). B Magnified image at 18 h post-infection. C. PLA dots per cell graphic, from 100 cells. Show the highest value at 18 h.p.i. mean 2.9 PLA dots per cell