Fig. 1

Immunofluorescence Assay of ZIKV infected cells. A Vero cells were infected with ZIKV and fixed at different times post-infection (p.i.). ZIKV pE was marked with mouse MAb and a secondary anti-mouse IgG Alexa 488 (red). Dynein HC was marked with rabbit polyclonal Ab and an anti-rabbit IgG Alexa 594 (green). Nuclei were stained with DAPI (blue). Split images showing the colocalization in a 12, 24, and 48 h time course. Mock cells are cells without infection fixed at 48 h. B Magnified immunofluorescence image at 12 h post-infection. C Magnified immunofluorescence image at 24 h post-infection.The lower panel of figure B and C shows the colocalization profiles of the sections marked with the white line. The green line is Dynein-HC and the red line is pE from the ZIKV. The colored areas are: Light yellow perinuclear section, blue is the nuclear area, magenta is the cytoplasm area, and the dotted line is the plasma membrane