Fig. 2

Minimal Differences in Nucleotide Frequency Between Clades, Over Time Across Structural Genes. Clades are represented by their Greek letter and corresponding color for line graphs: Alpha—α (red), Beta/Mu—β/μ (blue), Gamma/Lambda—γ/λ (black), Delta—δ (orange), and Omicron—ο (green). Within each box plot, orange bars represent the median nucleotide frequency of each clade distribution, red dots represent outliers, and black boxes represent the first and third quartiles (often hidden behind the orange median bar). A Spike gene nucleotide frequency distributions over select months. B Spike gene average nucleotide frequencies plotted over significantly large sampling months. C E gene nucleotide frequency distributions over select months. D E gene average nucleotide frequencies plotted over significantly large sampling months. E M gene nucleotide frequency distributions over select months. F M gene average nucleotide frequencies plotted over significantly large sampling months. G N gene nucleotide frequency distributions over select months. F N gene average nucleotide frequencies plotted over significantly large sampling months. All line graphs utilized the bold regions of the Table 1 clade distributions