A novel weevil-transmitted tymovirus found in mixed infection on hollyhock

Table 1 Primers used in this study

Name	Sequence	Use	References
CIFor	GGIVVIGTIGGIWSIGGIAARTCIAC	Potyvirus generic detection	[28]
CIRev	ACICCRTTYTCDATDATRTTIGTIGC	Potyvirus generic detection	[28]
TymZ-F	GGSCCMGTSAARAARTAYCA	Tymovirus generic detection	[29]
TymZ-R	GCCAGRTTGTARTCRGRGTTG	Tymovirus generic detection	[29]
CarF2b	GGRCTDGGDGTVCCNACTGA	Carlavirus generic detection	[30]
CarR	CCWCATYSRCTCSRCTWTGG	Carlavirus generic detection	[30]
TymoFinalF	CCCTACACCACGTCCCATTC	AYMV assembly gap & detection	This study
TymoFinalR	CAAGTGGGGTCGGTCAGAAG	AYMV assembly gap & detection
Seq5two	GATGGCCCAGGGAAGTTCTG	AYMV 5’ terminus
Tymo5closer	TCCGTGACCATCTCTGGAGT	AYMV 3’ terminus
AnchoredPolyA	A₂₂BNN	AYMV 3’ terminus
Carla_GLV_For	GGTACAGGAATTGGCTGGCT	GLV detection
Carla_GLV_Rev	TTCCAATACTGACGGCTCGG	GLV detection
Poty_Malva_Fwd	GAACCAACACGACCACTTGC	Novel potyvirus detection
Poty_Malva_Rv	AGTGTGGGTTGGCTGAAGTT	Novel potyvirus detection

I = inosine, Y = C/T, B = C/G/T, R = G/A, W = A/T, V = A/C/G, S = C/G and D = A/G/T

ISSN: 1743-422X