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Table 1 Primers and probes used in this study

From: Detection of porcine cytomegalovirus, a roseolovirus, in pig ovaries and follicular fluid: implications for somatic cells nuclear transfer, cloning and xenotransplantation

PCR assay

Accession number

Primer/probe

Sequence 5′–3′

Location (nucleotid number)

References

PCMV*

AF268040.2

PCMV-Fwd

GTT CTG GGA TTC CGA GGT TG

5074–5093

[13, 15,16,17]

PCMV-Rev

ACT TCG TCG CAG CTC ATC TGA

5036–5116

PCMV-Probe

6FAM-CAG GGC GGC GGT CGA GCT C-BHQ

5095–5113

pGAPDH

NM_001206359.1

pGAPDH-Fwd

ACA TGG CCT CCA AGG AGT AAG A

1083–1104

[14, 46]

pGAPDH-Rev

GAT CGA GTT GGG GCT GTG ACT

1188–1168

pGAPDH-Probe

HEX-CCA CCA ACC CCA GCA AGA GCA CGC-BHQ

1114–1137

  1. PCR Polymerase chain reaction, PCMV Porcine cytomegalovirus, pGAPDH Porcine glyceraldehyde-3-phosphate dehydrogenase, Fwd forward primer, Rev Reverse primer, nt nucleotide, 6FAM 6-carboxyfluorescein, BHQ Black hole quencher, HEX hexachlorofluorescein
  2. *The PCMV real-time PCR was modified and performed as duplex PCR.
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Virology Journal

ISSN: 1743-422X