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Table 4 Intra- and Inter-assay reproducibility of duplex qPCR for detecting HTLV-1 and RPPH1

From: Development and validation of a duplex real-time PCR for the rapid detection and quantitation of HTLV-1

No. of copies

HTLV-1

RPPH1

 

Intra-assay Ct values

Inter-assay Ct values

Intra-assay Ct values

Inter-assay Ct values

 

Mean SD

CV (%)

Mean SD

CV (%)

Mean SD

CV (%)

Mean SD

CV (%)

107copies/μL

11.70 ± 0.22

1.88

11.74 ± 0.35

3.06

11.92 ± 0.06

0.87

12.01 ± 0.10

0.48

103copies/μL

25.14 ± 0.08

0.3

25.18 ± 0.10

0.39

25.57 ± 0.18

1.31

25.51 ± 0.00

1.55

5 copies/μL

34.97 ± 0.32

0.92

34.99 ± 0.33

0.93

37.04 ± 0.35

1.95

36.94 ± 0.72

2.9

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Virology Journal

ISSN: 1743-422X