Table 3 The LoD of cell standards of dual fluorescent qPCR system
Cell concentrations (Hut102/Jurkat) (%) | PVL (copies/100 cells) | Positive results/total tests | Positive rate (%) |
|---|---|---|---|
20 | 22.92 | 20 | 100 |
4 | 4.584 | 20 | 100 |
0.8 | 0.9186 | 20 | 100 |
0.16 | 0.183 | 20 | 100 |
0.032 | 3.67 × 10–2 | 20 | 100 |
0.016 | 1.83 × 10–2 | 15/20 | 75 |
0.0064 | 7.33 × 10–3 | 5/20 | 25 |
0.0032 | 3.67 × 10–3 | 1/20 | 5 |
0 | 0 | 0 | 0 |