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Fig. 6 | Virology Journal

Fig. 6

From: Ethanol extract from Artemisia argyi leaves inhibits HSV-1 infection by destroying the viral envelope

Fig. 6

AEE inhibits HSV-1 early infection process. A AEE inhibited HSV-1 attachment. Vero cells were pre-cooled at 4 °C for 1 h and were infected with HSV-1 (MOI = 0.1) in the presence of AEE at 4 °C for 80 min. The cells were then incubated at 37 °C for 72 h and subjected to plaque assays. B AEE inhibited viral penetration. Pre-cooled Vero cells were treated with HSV-1 (MOI = 0.1) and AEE at 4 °C for 2 h, then incubated at 37 °C for another 10 min. After incubation, the bound but not entered virions were removed by alkaline PBS buffer (pH = 11.0). The cells were then subjected to plaque assay. C AEE directly inactivated viral particles. HSV-1 Virions were incubated with AEE at 37 °C for 2 h. After incubation, the drug-virus mixture was added to Vero cells to perform a plaque assay. The inhibitory rate was presented as the means ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 versus virus group, unpaired t test

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