Fig. 5

AEE inhibits HSV-2 and RV infection. A Vero cells were infected with HSV-2 (MOI = 0.1) for 2 h in the presence of AEE, and a viral plaque assay was performed (left). The inhibitory rate was calculated according to plaque numbers (right). B Vero cells were infected with HSV-2 (MOI = 0.1) in the presence of AEE for 24 h, and the total viral DNA was extracted to perform RT-qPCR. C MA-104 cells were infected with rotavirus (RV) (MOI = 0.1) for 72 h in the presence of AEE, and the virus titer was determined. Data were presented as the means ± SD (n = 3). D Vero cells were infected with H1N1 (MOI = 1 or 5) in the presence of AEE (10 μg/ml) at 37 °C for 24 h. Total RNA was extracted, and the mRNA expression of the H1N1 gene HA was detected by RT-qPCR. *P < 0.05, **P < 0.01, ***P < 0.001 versus virus group, unpaired t test