Table 1 Primers sequences for expression study of TLRs, cytokines and chemokines
Gene name | Primer sequence (5’-3’) | Location | Gene bank ID |
|---|---|---|---|
TLR-2 | F-GCAAACGCTGTTCTGCTCAG | 56–75 | NM_011905 |
|  | R-AGGCGTCTCCCTCTATTGTATT | 286–265 |  |
TLR-4 | F-AGGCACATGCTCTAGCACTAA | 287–307 | NM_021297 |
|  | R-AGGCTCCCCAGTTTAACTCTG | 368–348 |  |
TLR-9 | F-ATGGTTCTCCGTCGAAGGACT | 1–21 | NM_031178 |
|  | R-GAGGCTTCAGCTCACAGGG | 118–100 |  |
IFN-γ | F-ATGAACGCTACACACTGCATC | 1–21 | NM_008337 |
|  | R-CCATCCTTTTGCCAGTTCCTC | 182–162 |  |
IL-6 | F-CCAAGAGGTGAGTGCTTCCC | 462–481 | NM_031168 |
|  | R-CTGTTGTTCAGACTCTCTCCCT | 579–558 |  |
IL-17 | F-TCT GAT GCT GTT GCT GCT G | 87–105 | NM_010552.3 |
|  | R-ACG GTT AGA GGT AGT CTG AGG | 254–267 |  |
IL-21 | F-GGACCCTTGTCTGTCTGGTAG | 8–28 | NM_021782 |
|  | R-TGTGGAGCTGATAGAAGTTCAGG | 173–151 |  |
Foxp3 + regulatory T cells | F- GGC CCT TCT CCA GGA CAG A | 551–570 | NM_054039.2 |
|  | R-GCT GAT CAT GGC TGG GTT GT | 642–662 |  |
RANTES(CCL-5) | F-GCTGCTTTGCCTACCTCTCC | 98–117 | NM_013653 |
|  | R-TCGAGTGACAAACACGACTGC | 201–181 |  |
MCP-1(CCL-2) | F-TTAAAAACCTGGATCGGAACCAA | 260–282 | NM_01133 3 |
|  | R-GCATTAGCTTCAGATTTACGGGT | 380–358 |  |
β-actin | F-TGG AAT CCT GTG GCA TCC ATG AAA C | 885–909 | NM_007393.3 |
|  | R-TAA AAC GCA GCT CAG TAA CAG TCC G | 1209–1233 |  |