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Fig. 8 | Virology Journal

Fig. 8

From: Reactive oxygen species enhance rAAV transduction by promoting its escape from late endosomes

Fig. 8

Effects of cathepsins B or cathepsins L silencing on rAAV2 transduction. Hela cells were pretreated with CA-074, cathepsins L inhibitor for 4 h or transfected with 10 nM CTSB siRNA, CTSL siRNA or scrambled siRNA for 24 h, then transduced with rAAV2-GFP at 1000 vg/cell. A The representative images of Hela cells transfected with scrambled siRNA, CTSB siRNA or pretreated with CA-074 and then transduced with rAAV2-GFP for 48 h. B Quantification of rAAV2 transduction efficiency after CTSB siRNA or CA-074 pretreatment. C After treatment, the CTSB level was measured through WB assay. D Quantitative analysis of CTSB expression. E Quantitative analysis of vector genomes in late endosomes when rAAV2-GFP transduced Hela cells for 7 h, which was pretreated with CTSB siRNA or CA-074. F The representative images of Hela cells transfected with scrambled siRNA, CTSL siRNA, or pretreated with CTSL inhibitor and then transduced with rAAV2-GFP for 48 h. G Quantitation of transduction efficiency of rAAV2 after the cell was pretreated with CTSL siRNA or CTSL inhibitor. H After treatment, the CTSL level was measured through WB assay. I Quantitative analysis of CTSL expression. J Quantitative analysis of vector genomes in late endosomes when rAAV-GFP transduced Hela cells for 7 h pretreated with CTSL siRNA or cathepsins L inhibitor. All values shown are means ± SD (n = 3). ns p > 0.05; ****p < 0.0001

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