Fig. 7
From: Reactive oxygen species enhance rAAV transduction by promoting its escape from late endosomes
Effects of cathepsins B or cathepsins L overexpression on rAAV2 transduction. pcDNA3.1-CTSB, pcDNA3.1-CTSL, or pcDNA3.1 plasmid was transfected into HeLa cells for 24 h, then transduced with rAAV2-GFP at 500 vg/cell. A The representative images of pcDNA3.1 or pcDNA3.1- CTSB-transfected and rAAV2-GFP-transduced HeLa cells for 48 h. B Quantification of rAAV2 transduction efficiency after pcDNA3.1 or pcDNA3.1-CTSB pretreatment in cells. C After treatment for 24 h, the CTSB level was measured through WB assay. D Quantitative analysis of CTSB expression. E Quantitative analysis of vector genomes in late endosomes when rAAV-GFP transduced the pretreated Hela cells for 7 h. F The representative images of pcDNA3.1 or pcDNA3.1-CTSL-transfected and then rAAV2-GFP-transduced HeLa cells for 48 h. G Quantitation of transduction efficiency of rAAV2 after cell pretreated with pcDNA3.1 or pcDNA3.1-CTSL. H After treatment for 24 h, the CTSL level was measured through WB assay. I Quantitative analysis of CTSB expression. J Quantitative analysis of vector genomes in late endosomes when rAAV-GFP transduced pretreated Hela cells for 7 h. In addition, all data are represented by means ± SD (n = 3). ****p < 0.0001