Fig. 4

Depletion of ROS reduced rAAV2 transduction efficiency in Hela cells. Hela cell pretreated with shikonin, H₂O₂, or combined with NAC for 4 h, the medium was replaced, and rAAV2 was transduced. A Hela cells were treated with shikonin or combined with NAC for 4 h; DHE probes were used to measure ROS levels after 12-h pre-treatment. B Pretreated Hela cells with shikonin transduced with rAAV2-GFP vectors at 500 vg/cell. Transgene expression levels were detected under fluorescent microscopy 48 h after the transduction. C Statistical fluorescence intensity analysis with DHE fluorescent probe. D Statistical analyses of ss-rAAV2 transduction efficiency. E Hela cells treated with H₂O₂ or H₂O₂ combined with NAC for 4 h; ROS content was determined by DHE probes after 12 h of pre-treatment. F Pretreated Hela cells with H₂O₂ transduced with rAAV2-GFP vectors at 500 vg/cell. Fluorescent microscopy was conducted to obtain transgene levels 48 h following transduction. G Statistical fluorescence intensity analysis of DHE fluorescent probe. H Quantitative analyses of ss-rAAV2 transgene efficiency. All values indicated are means ± SD (n = 3). ^ns p > 0.05; **p < 0.01; ****p < 0.0001