Fig. 1
From: Reactive oxygen species enhance rAAV transduction by promoting its escape from late endosomes
Shikonin pretreatment increased ssAAV2-GFP or scAAV2-GFP transduction in 293 T or HeLa cells. A Cell viability results of 293 T exposed to 24-h shikonin treatment at different doses. Quantitation of transduction efficiency of ss-rAAV2 at 500 vg/cell (B) or sc-rAAV2 at 300 vg/cell (C) in 293 T cells treated with different doses of shikonin. The representative images of ss-rAAV2 (D) and sc-rAAV2- mediated E transgene expression in pretreated 293 T were measured using fluorescence microscopy after the infection for 48 h. F Cell viability results of Hela exposed to 24-h shikonin treatment at various doses. Quantitation of transduction efficiency of ss-rAAV2 at 500 vg/cell (G) or sc-rAAV2 at 300 vg/cell (H) in Hela cells were treated with different doses of shikonin. The representative images of ss-rAAV2 (I) and sc-rAAV2- mediated (J) transgene expression in pretreated Hela were measured using fluorescence microscopy after the infection for 48 h. Data are represented by means ± SD (n = 3). ns p > 0.05; **p < 0.01; ****p < 0.0001