Fig. 6

Evaluation of the in vitro RISC cleavage efficiency using TuMV-infected plants. a MYB33-230 RNA substrates were cleaved by AGO1-IP from mock, TuGR-, and TuGK-infected Col-0 plants. The numbers indicate the amounts of AGO1-IP that were used for cleavage. b The AGO1-IP amounts from mock, TuGR-, and TuGK-infected Col-0 plants were evaluated by western blotting with α AGO1 IgG to normalize the RISC cleavage efficiency. In: input; IP: immunoprecipitation; FT: flow through. c Normalized RISC cleavage efficiency of AGO1-IP from mock, TuGR-, and TuGK-infected Col-0 plants