Fig. 5

The AGO1 levels and evaluation of miRNA-mediated target cleavage in TuMV-infected plants. a Symptoms of TuGR- and TuGK-infected Col-0 plants at 12 dpi. Bar, 1 cm. The red arrowheads indicate the curing and serrated systemic leaves after TuGR infection. b Endogenous AGO1 and HC-Pro levels in mock, TuGR-, and TuGK-infected plants. The CP levels were used to confirm TuMV infection. RUBISCO (asterisk) was used as loading controls. c miRNA target gene expression of AGO1 (miR168), ARF16 (miR160), and MYB33 (miR159) in mock, TuGR-, and TuGK-infected plants. The bars represent the standard errors (n = 3). The statistical significance was assessed based on Student’s t test. ** indicates P values < 0.01