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Fig. 3 | Virology Journal

Fig. 3

From: Development of an assay system for the analysis of host RISC activity in the presence of a potyvirus RNA silencing suppressor, HC-Pro

Fig. 3

The functional studies of HC-Pro for AGO1 regulation. a Phenotypes of P1/HC-Pro plants. The photographs were obtained from 14-day-old seedlings. Bar, 0.2 cm. b T-DNA insertions of various P1/HC-Pro plants. The diagrammatic chromosomes were labeled with the T-DNA insertion positions and critical RNA silencing component genes. Bar, 100,000 bp. c AGO1 levels in various P1/HC-Pro plants. RUBISCO (asterisk) and tubulin were used as loading controls. d Immunoprecipitation for evaluating the interactions of AGO1 with various HC-Pros. The asterisks indicate the heavy chain of IgG. Tubulin was used as a loading control

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