Fig. 1From: Development of an assay system for the analysis of host RISC activity in the presence of a potyvirus RNA silencing suppressor, HC-Proα-AGO1 antibody generation. a Amino acid alignment of the N-termini of AGO1, AGO2, AGO4, and AGO10. The gray box indicates the N-terminal region of AGO1 for antigen. b Evaluation of the α-AGO1 antibody for detection of endogenous AGO1 in Col-0 plants. The null ago1-36 mutant serves as the negative control. The asterisk indicates tubulin, which was used as a loading controlBack to article page