Fig. 4
Effect of PTE on SA-β-Gal staining in HCMV-infected WI-38 cells. Before virus infection, the culture medium was replaced with 0.2% FBS for 48 h to synchronize cells in the G0 phase. Then, serum-starved WI-38 cells were infected with HCMV (MOI = 0.5) with or without PTE treatment (5 μM, 10 μM) at 5 dpi and submitted for SA-β-Gal staining according to the Kit instructions. (A) Representative images were selected from three independent experiments. (B) Quantitative analysis of SA-β-gal-positive cells out of the total cells from the average data obtained from three independent experiments. The sample without HCMV infection was used as a control. #P < 0.05 versus the control, ##P < 0.01 versus the control, ###P < 0.001 versus the control. **P < 0.01 versus the HCMV-only group