Fig. 1
From: Development of novel monoclonal antibodies against nsp12 of SARS-CoV-2
Development of mouse monoclonal antibodies against SARS-CoV-2 nsp12. A, B Structure of SARS-CoV-2 nsp12. The RdRP nsp12 comprises the NiRAN, interface, and the RdRP domains, which has the right-handed structure composed of fingers, palm, and thumb domains. The ribbon diagrams were cited from Protein Data Bank (PDB) (accession numbers 7BTF). NiRAN, the nidovirus RdRP-associated nucleotidyltransferase domain. The peptides (#1–3) used to immunize mice are shown. C Western blotting analysis using mouse monoclonal antibodies (mAb) against SARS-CoV-2 nsp12 (RdMab-2, -13, and -20). A FLAG-tagged nsp12 of SARS-CoV-2 transiently expressed in 293T cells was detected using an anti-FLAG antibody and RdMabs