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Table 1 Primers for uniplex RT-PCR assay and multiplex RT-PCR assay

From: Development of a multiplex RT-PCR assay for simultaneous detection of Lily symptomless virus, Lily mottle virus, Cucumber mosaic virus, and Plantago asiatica mosaic virus in Lilies

Virus

Primer

Primer sequence (5′–3′)

Products

Target gene

LSV

LSV-F

TGGCAACCACTGAACAAA

116 bp

(OP680588)

CP

LSV-R

GCTCACGCAAGCACACAT

LMoV

LMoV-F

CGCATTTCTCACATCTCGC

247 bp

(OP680591)

CP

LMoV-R

TCTTGGGTGGTCACCTTTC

CMV

CMV-F

CACATCTATCACCCTAAAGC

359 bp

(OP680590)

CP

CMV-R

TTGAATACACGAGGACGG

PlAMV

PlAMV-F

ACCAACTCTGGGCCTTACA

525 bp

(OP680589)

RdRp

PlAMV-R

TCTGGGTGCTTTCCGTCA