Table 1 Histopathology evaluation of C57BL/6 mice treated with AYA2012004_L, ODN 1826 and DPBS
Mouse tissues | AYA2012004_L | DPBS | ODN 1826 |
|---|---|---|---|
Lungs | A section of both lungs appears normal except for occasional areas of atelectasis likely introduced during dissection. Of note, no necrosis, inflammatory infiltrates, or hemorrhages are seen. | A section of both lungs shows minute areas of atelectasis as well as minute hemorrhages. These changes are likely due to tissue handling at the time of dissection. Notably, no necrosis or inflammatory infiltrates are seen. | A section of both lungs shows minute areas of atelectasis as well as minute hemorrhages. These changes are likely due to tissue handling at the time of dissection. Notably, no necrosis or inflammatory infiltrates are seen. |
Brain | A sagittal section through the brain reveals normal brain architecture without areas of infarction, inflammation, or gliosis. The cortical laminations are intact, and the hippocampal cornu ammonis (CA system) and dentate gyrus are normal. The meninges and ventricular system appear unremarkable. | A sagittal section through the brain reveals multiple clear vacuoles predominately within the white matter of the brainstem and the cerebellum. These vacuoles do not appear confined to certain cells and are not involved by an associated inflammatory infiltrate. Given their random distribution within the posterior brain structures, it is reasonable to hypothesize that these vacuoles may be artifactual and due to technical manipulation during dissection. Aside from these vacuoles, the remainder of the brain is without areas of infarction, inflammation, or gliosis. The cortical laminations are intact, and the hippocampal cornu ammonis (CA system) and dentate gyrus are normal. The meninges and ventricular system appear unremarkable. | A sagittal section through the brain reveals multiple clear vacuoles predominately within the white matter of the brainstem and the cerebellum. These vacuoles do not appear confined to certain cells and are not involved by an associated inflammatory infiltrate. Given their random distribution within the posterior brain structures, it is reasonable to hypothesize that these vacuoles may be artifactual and due to technical manipulation during dissection. Aside from these vacuoles, the remainder of the brain is without areas of infarction, inflammation, or gliosis. The cortical laminations are intact, and the hippocampal cornu ammonis (CA system) and dentate gyrus are normal. The meninges and ventricular system appear unremarkable. |
Kidneys | Sections of both the left and right kidneys show no histopathologic abnormalities aside from rare, mildly dilated tubules and parenchymal vessels. The glomeruli are intact and not sclerotic. The renal tubules appear healthy and are without evidence of acute tubular necrosis. A minute intraluminal cast in a collecting duct is identified, which is likely of no significance. | Sections of both the left and right kidneys show no histopathologic abnormalities aside from rare, mildly dilated tubules and parenchymal vessels. The glomeruli are intact and not sclerotic. The renal tubules appear healthy and are without evidence of acute tubular necrosis. | Sections of both the left and right kidneys show no histopathologic abnormalities aside from rare, mildly dilated tubules and parenchymal vessels. The glomeruli are intact and not sclerotic. The renal tubules appear healthy and are without evidence of acute tubular necrosis. |
Heart | A section of the heart shows no histopathologic abnormalities. Notably, no areas of necrosis, infarction, inflammation, or fibrosis are identified. The valves appear normal. The ventricular and atrial septa are intact and appear normal. | A section of the heart shows no histopathologic abnormalities. Notably, no areas of necrosis, infarction, inflammation, or fibrosis are identified. The valves appear normal. The ventricular and atrial septa are intact and appear normal. | A section of the heart shows no histopathologic abnormalities. Notably, no areas of necrosis, infarction, inflammation, or fibrosis are identified. The valves appear normal. The ventricular and atrial septa are intact and appear normal. |
Lymph node | A section through this tissue reveals unremarkable striated muscle. No lymphoid tissue is seen microscopically. | A section through the lymph node revealed reveals unremarkable lymphoid tissue without germinal-center formation. | A section through the lymph node revealed reveals unremarkable lymphoid tissue without germinal-center formation |
Spleen | A section through the spleen reveals no histopathologic abnormalities, with good preservation of the red and white pulp. No germinal-center formation is noted | A section through the spleen reveals no histopathologic abnormalities, with good preservation of the red and white pulp. No germinal-center formation is seen. | A section through the spleen reveals no histopathologic abnormalities, with good preservation of the red and white pulp. No germinal-center formation is seen. |
Liver | A section through the liver reveals normal hepatic architecture without evidence of necrosis, inflammation, or fibrosis. The portal tracts are well preserved. The hepatocytes demonstrate cytoplasmic glycogen accumulation, the amount of which is normal for ad libitum-fed mice. | A section through the liver reveals normal hepatic architecture without evidence of necrosis, inflammation, or fibrosis. The portal tracts are well preserved. The hepatocytes demonstrate cytoplasmic glycogen accumulation, the amount of which is normal for ad libitum-fed mice. | A section through the liver reveals normal hepatic architecture without evidence of necrosis, inflammation, or fibrosis. The portal tracts are well preserved. The hepatocytes demonstrate cytoplasmic glycogen accumulation, the amount of which is normal for ad libitum-fed mice. |