Fig. 4

Concentration of AYA2012004_L in mouse lungs. C57BL/6J mice were treated with 200 ul of DPBS buffer containing 1 mM MgCl\(_{2}\) in the absence or presence of AYA2012004_L (10 uM) via bronchoalveolar lavage. Mice were then sacrificed at 1, 4, 8, 12, 24 and 48 hrs post-treatment; and lungs were collected and processed for nucleic acid isolation. AYA2012004_L was detected in mice lungs by real-time PCR (RT-PCR) assays. Number of aptamer molecules per mg total DNA was calculated as indicated in the inset picture by plotting a standard curve between \(\Delta\)Ct values and total DNA concentration (pM). Data are representative over two independent experiments