Fig. 2
From: Identification of a neutralizing linear epitope within the VP1 protein of coxsackievirus A10
Peptide-specific IgG antibody levels in the sera obtained from immunized mice. Five adult female BALB/c mice received priming injection at week 0 and boosting injection at weeks 2 and 4 with PBS, irrelevant peptide, EP3, EP4, or EP5, respectively. The end-point dilution titers were tested by peptide ELISA using EP3 (A), EP4 (B), or EP5 (C) peptide as the corresponding antigen. Anti-PBS sera served as blank control, and anti-irrelevant peptide sera served as the negative control (NC). The data are presented as mean ± standard deviation (SD) of at least triplicate wells, and the means of IgG titers were compared using a student's two-tailed t-test. **p < 0.01, ****p < 0.0001