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Fig. 2 | Virology Journal

Fig. 2

From: Genetic characterization and pathogenicity of a Eurasian avian-like H1N1 swine influenza reassortant virus

Fig. 2

Identification of A/swine/Heilongjiang/GN/2020 strain. A Identification of the isolates by RT-PCR using specific primer of M gene. B Identification of the isolates by IFA using NP monoclonal antibody of IAV. C Identification of virus by WB using NP monoclonal antibody of IAV. D TEM shows features of an orthomyxovirus particle by negative stain (Bar = 100 nm). E Identification of whole-genome by RT-PCR using segment-specific primers for eight genes. P3, A/swine/Heilongjiang/GN/2020 virus was proliferated with 3 passages. NC, negative control. anti-NP. IFA, Indirect immunofluorescence assay. WB, Western blot assay. TEM: Transmission electron microscopic. PB2, PB1, PA, HA, NP, NA, M and NS were protein of IAV.

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