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Fig. 3 | Virology Journal

Fig. 3

From: Development of an indirect ELISA using a novel linear epitope at the C-terminal region of the VP2 protein to specifically detect antibodies against Senecavirus A

Fig. 3

Results of checkerboard titration assays used to optimize peptide concentrations and serum dilution. a Standard positive (P) and standard negative (N) control serum samples were serially diluted 2-fold (1:10, 1:20, 1:40, 1:80), and the coating concentration of the identified peptide was varied from 25ng/well to 200ng/well. b When the conditions were a coating concentration of the peptide of 50 ng/well and a standard control serum sample (P and N) dilution ratio of 1:80, and the best signal-to-noise ratio (P/N value) of VP2-epitp-ELISA was 17

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Virology Journal

ISSN: 1743-422X